|
KMID : 1137020180290030038
|
|
Journal of Gynecologic Oncology
2018 Volume.29 No. 3 p.38 ~ p.38
|
|
|
E2/E6 ratio and L1 immunoreactivity as biomarkers to determine HPV16-positive high-grade squamous intraepithelial lesions (CIN2 and 3) and cervical squamous cell carcinoma
|
|
Choi Youn-Jin
Lee Ah-Won
Kim Tae-Jung
Jin Hyun-Tak
Park Jong-Sup
Lee Sung-Jong
|
|
|
Abstract
|
|
|
Objective: Human papillomavirus (HPV) 16 is the most carcinogenic HPV genotype. We investigated if HPV16 L1 capsid protein and E2/E6 ratio, evaluated by cervical cytology, may be used as biomarkers of ≥cervical intraepithelial neoplasia (CIN) 2 lesions.
Methods: Cervical specimens were obtained from 226 patients with HPV16 single infection. Using cytology specimen, L1 capsid protein and E2/E6 ratio were detected and the results were compared with those of the conventional histologic analysis of cervical tissues (CIN1?3 and squamous cell carcinoma [SCC]) to evaluate the association.
Results: The L1 positivity of CIN2/3 was significantly lower than that of normal cervical tissue (p<0.001) and SCC demonstrated significantly lower L1 positivity than CIN1 (p<0.001). The mean E2/E6 ratios of specimens graded as SCC (0.356) and CIN2/3 (0.483) were significantly lower than those of specimens graded as CIN1 (0.786) and normal (0.793) (p<0.05). We observed that area under the receiver operating characteristic curve (AUC) for E2/E6 ratio (0.844; 95% confidence interval [CI]=0.793?0.895) was higher than that for L1 immunochemistry (0.636; 95% CI=0.562?0.711). A combination of E2/E6 ratio and L1 immunocytochemistry analyses showed the highest AUC (0.871; 95% CI=0.826?0.917) for the prediction of ≥CIN2 lesions.
Conclusion: To our knowledge, this is the first study to validate HPV L1 capsid protein expression and decreased HPV E2/E6 ratio as valuable predictive markers of ≥CIN2 cervical lesions. Cervical cytology may be analyzed longitudinally on an outpatient basis with noninvasive procedures as against invasive conventional histologic analysis.
|
|
|
KEYWORD
|
|
|
Cervical Intraepithelial Neoplasia, Uterine Cervical Neoplasms, Human Papillomavirus Type 16 L1 Protein, Immunocytochemistry, Viral Integration
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
    
|